Hotspot

rbcL-accD

LSC · 56296-56992 · 697 bp

Back to Siphocranion

Pi 0.0131

MarkerSeek score 66.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 697
variable_sites: 9
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	CATACCCGCGTTCTAACTCC	50.0	55.0	58.0	58.2	850-854 bp	1.0	80.2
rbcL-accD_p2	CGTGAAGCTTGCAAATGGAG	CCATACCCGCGTTCTAACTC	50.0	55.0	58.0	58.2	851-855 bp	1.0	80.2
rbcL-accD_p3	CGTGAAGCTTGCAAATGGAG	ATACCCGCGTTCTAACTCCT	50.0	50.0	58.0	58.2	849-853 bp	1.0	79.6
rbcL-accD_p4	CCGCTTGTGAGGTATGGAAA	CATACCCGCGTTCTAACTCC	50.0	55.0	57.9	58.2	816-820 bp	1.0	79.6
rbcL-accD_p5	CCGCTTGTGAGGTATGGAAA	CCATACCCGCGTTCTAACTC	50.0	55.0	57.9	58.2	817-821 bp	1.0	79.6