Hotspot

trnF-GAA-ndhJ

LSC · 47818-48451 · 634 bp

Back to Siphocranion

Pi 0.00946

MarkerSeek score 64.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 6
pi: 0.00946
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	774 bp	1.0	80.0
trnF-GAA-ndhJ_p2	GAGGGTGGGATGCTACATTG	TTGGATAGGATGGCCTTTGC	55.0	50.0	58.0	57.9	803 bp	1.0	79.8
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	738 bp	1.0	79.7
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	TTGGATAGGATGGCCTTTGC	55.0	50.0	57.9	57.9	762 bp	1.0	79.0
trnF-GAA-ndhJ_p5	GGAAATGGTCGGGATAGCTC	TTGGATAGGATGGCCTTTGC	55.0	50.0	57.8	57.9	784 bp	1.0	78.7