Hotspot

ycf3-trnS-GGA

LSC · 43974-44823 · 850 bp

Back to Siphocranion

Pi 0.00947

MarkerSeek score 63.30

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 8
pi: 0.00947
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TGCCTCCTTTTCTCCTGAAG	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1017-1022 bp	1.0	73.3
ycf3-trnS-GGA_p2	GCCTCCTTTTCTCCTGAAGT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	57.1	58.1	1016-1021 bp	1.0	73.3
ycf3-trnS-GGA_p3	TGCCTCCTTTTCTCCTGAAG	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1048-1053 bp	1.0	73.1
ycf3-trnS-GGA_p4	GCCTCCTTTTCTCCTGAAGT	AAGTGAAGGTGCGGAAAGAG	50.0	50.0	57.1	58.1	1047-1052 bp	1.0	73.1
ycf3-trnS-GGA_p5	TGCCTCCTTTTCTCCTGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.1	57.8	988-993 bp	1.0	72.6