Hotspot

trnY-GUA-trnE-UUC

LSC · 30126-30211 · 86 bp

Back to Siphocranion

Pi 0.0123

MarkerSeek score 63.40

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 86
variable_sites: 1
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CCAGCGTAGACATATTGCCA	CATAGTATGAGGGCGGTTGG	50.0	55.0	57.8	57.8	246-251 bp	1.0	78.7
trnY-GUA-trnE-UUC_p2	ACCAGCGTAGACATATTGCC	CATAGTATGAGGGCGGTTGG	50.0	55.0	57.8	57.8	247-252 bp	1.0	78.6
trnY-GUA-trnE-UUC_p3	CCAGCGTAGACATATTGCCA	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.8	58.3	190-195 bp	1.0	78.5
trnY-GUA-trnE-UUC_p4	CCAGCGTAGACATATTGCCA	ATAGTATGAGGGCGGTTGGT	50.0	50.0	57.8	58.2	245-250 bp	1.0	78.4
trnY-GUA-trnE-UUC_p5	ACCAGCGTAGACATATTGCC	ATAGTATGAGGGCGGTTGGT	50.0	50.0	57.8	58.2	246-251 bp	1.0	78.4