Hotspot

rpoB-trnC-GCA

LSC · 26736-27894 · 1159 bp

Back to Siphocranion

Pi 0.0156

MarkerSeek score 67.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 18
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GACATTGCCTCGTTTGCATC	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.1	58.0	1265-1271 bp	1.0	80.5
rpoB-trnC-GCA_p2	GACATTGCCTCGTTTGCATC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1244-1250 bp	1.0	80.1
rpoB-trnC-GCA_p3	TAGACATTGCCTCGTTTGCA	TTTTGGTGATTCAGGCGACA	45.0	45.0	57.8	58.0	1267-1273 bp	1.0	79.9
rpoB-trnC-GCA_p4	TAGACATTGCCTCGTTTGCA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.8	58.1	1246-1252 bp	1.0	79.5
rpoB-trnC-GCA_p5	GCCAAACCCTGATCGATGAA	TTTTGGTGATTCAGGCGACA	50.0	45.0	58.2	58.0	1331-1337 bp	1.0	79.2