Hotspot

psbI-trnS-GCU

LSC · 7849-8092 · 244 bp

Back to Siphocranion

Pi 0.0383

MarkerSeek score 60.00

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 244
variable_sites: 7
pi: 0.0383
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.75
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCCGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.3	58.0	287-340 bp	1.0	80.5
psbI-trnS-GCU_p2	GTAATCCCGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.3	58.0	288-341 bp	1.0	80.5
psbI-trnS-GCU_p3	GTAATCCCGGACGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.3	58.4	285-338 bp	1.0	77.6
psbI-trnS-GCU_p4	GTAATCCCGGACGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.3	58.4	292-345 bp	1.0	77.4
psbI-trnS-GCU_p5	CGTAATCCCGGACGTGAAG	TGGACTAAAGCGTCGGATTG	57.9	50.0	58.0	58.0	288-341 bp	1.0	77.4