Hotspot

trnH-GUG-psbA

LSC · 83-316 · 234 bp

Back to Siphocranion

Pi 0.0858

MarkerSeek score 72.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 234
variable_sites: 20
pi: 0.0858
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	683-684 bp	1.0	81.2
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GAGTGGGAGCTTAGTTTCCG	55.0	55.0	58.1	58.0	927-928 bp	1.0	80.8
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	941-942 bp	1.0	80.7
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	940-941 bp	1.0	80.7
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GAGTGGGAGCTTAGTTTCCG	50.0	55.0	57.9	58.0	942-943 bp	1.0	80.7