Hotspot

rps16

LSC · 5253-6348 · 1096 bp

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Pi 0.0134

MarkerSeek score 55.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1096
variable_sites: 33
pi: 0.0134
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACT	GTCTATGTACCGAGCGAACC	45.0	55.0	57.9	57.9	1558-1591 bp	1.0	82.9
rps16_p2	ACTTGTGTTGGATTGGCACT	CCGAGCGAACCAATGACTAT	45.0	50.0	57.9	57.8	1549-1582 bp	1.0	82.0
rps16_p3	ACTTGTGTTGGATTGGCACT	TCTATGTACCGAGCGAACCA	45.0	50.0	57.9	58.2	1557-1590 bp	1.0	81.8
rps16_p4	ACTTGTGTTGGATTGGCACT	TTCGGGAAGTTGGAAATGGG	45.0	50.0	57.9	58.1	1829-1862 bp	0.857143	77.3
rps16_p5	TGTTGGATTGGCACTGCATA	TTCGGGAAGTTGGAAATGGG	45.0	50.0	57.8	58.1	1824-1857 bp	0.857143	76.2