Hotspot

trnL-UAG-ccsA

SSC · 112493-112608 · 116 bp

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Pi 0.0183

MarkerSeek score 63.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 116
variable_sites: 5
pi: 0.0183
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	TGAGTGAATATATGCTCTAAAGTTGA	50.0	30.8	57.9	56.3	211-223 bp	1.0	45.6
trnL-UAG-ccsA_p2	GGTAGACACGCTGCTCTTAG	TGAGTGAATATATGCTCTAAAGTTGA	55.0	30.8	57.8	56.3	213-225 bp	1.0	45.5
trnL-UAG-ccsA_p3	GTAGACACGCTGCTCTTAGG	TGAGTGAATATATGCTCTAAAGTTGA	55.0	30.8	57.8	56.3	212-224 bp	1.0	45.5
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	TGAGTGAATATATGCTCTAAAGTTGA	50.0	30.8	58.0	56.3	220-232 bp	1.0	45.4
trnL-UAG-ccsA_p5	GTGAAATCGGTAGACACGCT	TGAGTGAATATATGCTCTAAAGTTGA	50.0	30.8	58.0	56.3	221-233 bp	1.0	45.3