Hotspot

ndhF-rpl32

SSC · 110429-111462 · 1034 bp

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Pi 0.0196

MarkerSeek score 58.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1034
variable_sites: 42
pi: 0.0196
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACGCATATTGATATATCTGTTCC	TTTGATATCGAAGTACGTTTTT	40.0	27.3	57.8	52.2	1081-1118 bp	1.0	45.2
ndhF-rpl32_p2	CCACGCATATTGATATATCTGTTCC	TTTTGATATCGAAGTACGTTTT	40.0	27.3	57.8	52.2	1082-1119 bp	1.0	45.2
ndhF-rpl32_p3	CCACGCATATTGATATATCTGTTCC	TTTTTGATATCGAAGTACGTTT	40.0	27.3	57.8	52.2	1083-1120 bp	1.0	45.2
ndhF-rpl32_p4	CCACGCATATTGATATATCTGTTCC	TTTTGATATCGAAGTACGTTTTT	40.0	26.1	57.8	53.1	1082-1119 bp	1.0	45.2
ndhF-rpl32_p5	CCACGCATATTGATATATCTGTTCC	TTTTTGATATCGAAGTACGTTTT	40.0	26.1	57.8	53.1	1083-1120 bp	1.0	45.2