Hotspot

rpl14-rpl16

LSC · 80195-80311 · 117 bp

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Pi 0.0288

MarkerSeek score 63.20

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 6
pi: 0.0288
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	TCGACGATTGCTAGCTCCTA	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	395-409 bp	1.0	83.1
rpl14-rpl16_p2	GCGTCTTTGATCACAGCAAC	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	439-453 bp	1.0	82.7
rpl14-rpl16_p3	TCGACGATTGCTAGCTCCTA	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	521-535 bp	1.0	82.3
rpl14-rpl16_p4	CGCACCAAAAACTCGAGTTC	GGTTACAGTAAGACCCGCAG	50.0	55.0	58.0	58.0	605-619 bp	1.0	82.0
rpl14-rpl16_p5	CGCACCAAAAACTCGAGTTC	ATACGCTCTTCAGGCACTTG	50.0	50.0	58.0	58.0	731-745 bp	1.0	81.7