Hotspot

accD-psaI

LSC · 58270-58925 · 656 bp

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Pi 0.0266

MarkerSeek score 64.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 656
variable_sites: 41
pi: 0.0266
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAGAAGGGCTTATTCGATC	GGGACCAAAACAGAGGGAAA	47.6	50.0	57.2	57.6	789-846 bp	1.0	69.5
accD-psaI_p2	TCCAGAAGGGCTTATTCGATC	AAAGGGACCAAAACAGAGGG	47.6	50.0	57.2	57.6	792-849 bp	1.0	69.4
accD-psaI_p3	CCAGAAGGGCTTATTCGATCT	GGGACCAAAACAGAGGGAAA	47.6	50.0	57.2	57.6	788-845 bp	1.0	69.4
accD-psaI_p4	TTCCAGAAGGGCTTATTCGA	GGGACCAAAACAGAGGGAAA	45.0	50.0	56.2	57.6	790-847 bp	1.0	69.4
accD-psaI_p5	CCAGAAGGGCTTATTCGATCT	AAAGGGACCAAAACAGAGGG	47.6	50.0	57.2	57.6	791-848 bp	1.0	69.3