Hotspot

trnF-GAA-ndhJ

LSC · 47842-48614 · 773 bp

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Pi 0.0151

MarkerSeek score 56.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 26
pi: 0.0151
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	58.1	53.8	790-829 bp	1.0	46.6
trnF-GAA-ndhJ_p2	AATCCTCGTGTCACCAGTTC	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.5	53.8	793-832 bp	1.0	46.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATGAAATACAAGATGCTCATTGA	50.0	30.4	58.1	54.4	791-830 bp	1.0	46.6
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGCTCCCAATTTTTATGAAATACA	50.0	29.2	58.1	55.3	805-844 bp	1.0	46.5
trnF-GAA-ndhJ_p5	TGGTAGAGCAGAGGACTGAA	TGAAATACAAGATGCTCATTGA	50.0	31.8	57.4	53.8	813-852 bp	1.0	46.5