Hotspot

trnT-UGU-trnL-UAA

LSC · 46235-46866 · 632 bp

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Pi 0.0218

MarkerSeek score 68.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 632
variable_sites: 32
pi: 0.0218
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	644-713 bp	1.0	84.7
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	638-707 bp	1.0	83.4
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	55.0	55.0	57.9	58.6	643-712 bp	1.0	81.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	TCCGTAGCGTCTACCGATTT	55.0	50.0	57.9	58.6	645-714 bp	1.0	81.7
trnT-UGU-trnL-UAA_p5	ATGCGATGCTCTAACCTCTG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.5	58.2	646-715 bp	1.0	81.6