Hotspot

atpA-trnR-UCU

LSC · 29636-29751 · 116 bp

Back to Sinosenecio

Pi 0.0511

MarkerSeek score 71.90

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 116
variable_sites: 13
pi: 0.0511
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	TACTTCAGGAACAAGCAGCC	GCACTGAAAAGCGTCCATTG	50.0	50.0	57.8	58.3	180-237 bp	1.0	84.8
atpA-trnR-UCU_p2	TACTTCAGGAACAAGCAGCC	AGCACTGAAAAGCGTCCATT	50.0	45.0	57.8	58.4	181-238 bp	1.0	84.1
atpA-trnR-UCU_p3	TACTTCAGGAACAAGCAGCC	TAGCACTGAAAAGCGTCCAT	50.0	45.0	57.8	57.5	182-239 bp	1.0	83.5
atpA-trnR-UCU_p4	TACTTCAGGAACAAGCAGCC	ATAGCACTGAAAAGCGTCCA	50.0	45.0	57.8	57.5	183-240 bp	1.0	83.4
atpA-trnR-UCU_p5	CTTCAGGAACAAGCAGCCTA	GCACTGAAAAGCGTCCATTG	50.0	50.0	57.5	58.3	178-235 bp	1.0	82.5