Hotspot

psbI-trnS-GCU

LSC · 8501-8638 · 138 bp

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Pi 0.0348

MarkerSeek score 67.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 138
variable_sites: 11
pi: 0.0348
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCAGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	249-256 bp	1.0	78.0
psbI-trnS-GCU_p2	CGTAATCCAGGACGTGAAGA	CGAGTTATTCGTACCGAGGG	50.0	55.0	57.1	57.6	197-204 bp	1.0	77.3
psbI-trnS-GCU_p3	CGTAATCCAGGACGTGAAGAA	ATTGGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.1	249-256 bp	1.0	75.6
psbI-trnS-GCU_p4	CCTATCCAATGATCCAGGACG	ATTGGGAGAGATGGCTGAGT	52.4	50.0	57.7	58.1	268-275 bp	1.0	74.4
psbI-trnS-GCU_p5	CGTAATCCAGGACGTGAAGA	GATGGCTGAGTGGACGAAAG	50.0	55.0	57.1	58.6	240-247 bp	1.0	74.0