Hotspot

trnfM-CAU

LSC · 38717-38773 · 57 bp

Back to Sinosasa

Pi 0.0175

MarkerSeek score 62.60

Primer available yes

Label trnfM-CAU

Feature Metadata

label_name: trnfM-CAU
feature_type: tRNA
region: LSC
length_bp: 57
variable_sites: 1
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU_p1	AGAAGGAATATGGAGCGGGT	TTATCCACTAGTTCCCCGCT	50.0	50.0	58.2	57.8	116 bp	1.0	77.8
trnfM-CAU_p2	AGAAGGAATATGGAGCGGGT	TATGGTAGAGGGGCGTATCC	50.0	55.0	58.2	57.8	145 bp	1.0	77.2
trnfM-CAU_p3	AGAAGGAATATGGAGCGGGT	TATCCACTAGTTCCCCGCTC	50.0	55.0	58.2	58.3	115 bp	1.0	76.8
trnfM-CAU_p4	AGGAATATGGAGCGGGTAGT	TTATCCACTAGTTCCCCGCT	50.0	50.0	57.6	57.8	113 bp	1.0	76.1
trnfM-CAU_p5	AGAAGGAATATGGAGCGGGT	GCGTATCCAACCCCCTTTTA	50.0	50.0	58.2	57.6	133 bp	1.0	75.7