Hotspot

trnK-UUU

LSC · 1377-1409 · 33 bp

Back to Sinosasa

Pi 0.0303

MarkerSeek score 69.90

Primer available yes

Label trnK-UUU

Feature Metadata

label_name: trnK-UUU
feature_type: tRNA
region: LSC
length_bp: 33
variable_sites: 1
pi: 0.0303
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	TACCAATGTCAACCAAGCCA	AAATGCAAGCACGATTTGGG	45.0	45.0	57.3	57.9	230 bp	1.0	74.4
trnK-UUU_p2	ACCAATGTCAACCAAGCCAG	AAATGCAAGCACGATTTGGG	50.0	45.0	58.7	57.9	229 bp	1.0	74.3
trnK-UUU_p3	TACCAATGTCAACCAAGCCA	CATAGGGAAAGTCGTGTGCA	45.0	50.0	57.3	57.9	255 bp	1.0	74.2
trnK-UUU_p4	TACCAATGTCAACCAAGCCA	ACATAGGGAAAGTCGTGTGC	45.0	50.0	57.3	57.9	256 bp	1.0	74.2
trnK-UUU_p5	ACCAATGTCAACCAAGCCAG	CATAGGGAAAGTCGTGTGCA	50.0	50.0	58.7	57.9	254 bp	1.0	74.2