Hotspot

rps16

LSC · 4517-5607 · 1091 bp

Back to Sinosasa

Pi 0

MarkerSeek score 27.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1091
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGACATAAATCCAGT	TGCAATGAAATTAGAGGAATGT	45.0	31.8	57.2	54.0	1238 bp	1.0	40.0
rps16_p2	TGGCACGACATAAATCCAGT	TGCAATGAAATTAGAGGAATGTTTG	45.0	32.0	57.2	56.9	1238 bp	1.0	40.0
rps16_p3	TGGCACGACATAAATCCAGT	GCAATGAAATTAGAGGAATGTTTGT	45.0	32.0	57.2	56.9	1237 bp	1.0	40.0
rps16_p4	TGGCACGACATAAATCCAGT	TGCAATGAAATTAGAGGAATGTT	45.0	30.4	57.2	54.8	1238 bp	1.0	40.0
rps16_p5	TGGCACGACATAAATCCAGT	TTGCAATGAAATTAGAGGAATGT	45.0	30.4	57.2	54.8	1239 bp	1.0	40.0