Hotspot

rpl16

LSC · 80452-81990 · 1539 bp

Back to Sinosasa

Pi 0.0013

MarkerSeek score 59.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1539
variable_sites: 2
pi: 0.0013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTATGCAAAAGGATGAAAGA	AGCCTTATTTTTCCTGAAATCCC	33.3	39.1	52.8	57.1	1722 bp	1.0	40.1
rpl16_p2	TCTATGCAAAAGGATGAAAGA	GCCTTATTTTTCCTGAAATCCCT	33.3	39.1	52.8	57.1	1721 bp	1.0	40.1
rpl16_p3	TCTATGCAAAAGGATGAAAGA	TGACAAGAGCCTTATTTTTCCTG	33.3	39.1	52.8	57.0	1729 bp	1.0	40.1
rpl16_p4	TCTATGCAAAAGGATGAAAGAA	AGCCTTATTTTTCCTGAAATCCC	31.8	39.1	53.7	57.1	1722 bp	1.0	40.1
rpl16_p5	TCTATGCAAAAGGATGAAAGAA	GCCTTATTTTTCCTGAAATCCCT	31.8	39.1	53.7	57.1	1721 bp	1.0	40.1