Hotspot

atpI-atpH

LSC · 33820-34651 · 832 bp

Back to Sinosasa

Pi 0

MarkerSeek score 58.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	967-968 bp	1.0	74.3
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	900-901 bp	1.0	74.2
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	922-923 bp	1.0	74.2
atpI-atpH_p4	TAGCCGCAGCCTATATAGGT	ATAACGGAAGCAGCAGCAAT	50.0	45.0	57.5	58.2	907-908 bp	1.0	73.9
atpI-atpH_p5	ATTTTTGCAACGTTAGCCGC	GCAGTACCTTGACCAACTCC	45.0	55.0	58.6	58.2	980-981 bp	1.0	73.9