Hotspot

trnD-GUC-psbM

LSC · 17542-18574 · 1033 bp

Back to Sinosasa

Pi 0.00291
MarkerSeek score 60.70
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
1033
variable_sites
3
pi
0.00291
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCA 50.0 40.0 58.5 54.5 1115-1118 bp 1.0 48.5
trnD-GUC-psbM_p2 TAGGTATGCCATACACCCCG TGCGAGAATATTGACTTCCA 55.0 40.0 58.7 54.5 1147-1150 bp 1.0 47.0
trnD-GUC-psbM_p3 TTCAATTGGTCAGAGCACCG ATGCGAGAATATTGACTTCCA 50.0 38.1 58.5 55.1 1116-1119 bp 1.0 45.4
trnD-GUC-psbM_p4 TTCAATTGGTCAGAGCACCG TGCGAGAATATTGACTTCCAT 50.0 38.1 58.5 55.1 1115-1118 bp 1.0 45.4
trnD-GUC-psbM_p5 GTTCAATTGGTCAGAGCACC TGCGAGAATATTGACTTCCA 50.0 40.0 57.0 54.5 1116-1119 bp 1.0 44.3