Hotspot

psbK-psbI

LSC · 7769-8172 · 404 bp

Back to Sinosasa

Pi 0.00248

MarkerSeek score 52.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 1
pi: 0.00248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	548 bp	1.0	78.3
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	545 bp	1.0	76.2
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	546 bp	1.0	76.2
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	547 bp	1.0	76.2
psbK-psbI_p5	AAATTGCCCGAAGCTTATGC	TTTTTACTCCTCACGCCCAG	45.0	50.0	57.4	57.8	632 bp	1.0	74.0