Hotspot

petD

LSC · 80339-81595 · 1257 bp

Back to Sinojackia

Pi 0.000265

MarkerSeek score 46.60

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1257
variable_sites: 1
pi: 0.000265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ATATTTCCCTTCAACCCCGC	TGGCTCGATCCAGAATTGAA	50.0	45.0	57.9	56.9	1451-1452 bp	1.0	70.9
petD_p2	CCCTTCAACCCCGCAATATT	TGGCTCGATCCAGAATTGAA	50.0	45.0	58.2	56.9	1445-1446 bp	1.0	69.7
petD_p3	ATATTTCCCTTCAACCCCGC	GGGAGAATTCACTTTGAAGCG	50.0	47.6	57.9	57.5	1414-1415 bp	1.0	67.8
petD_p4	ATATTTCCCTTCAACCCCGC	TGGCTCGATCCAGAATTGAAT	50.0	42.9	57.9	57.4	1451-1452 bp	1.0	67.0
petD_p5	CCCTTCAACCCCGCAATATT	GGGAGAATTCACTTTGAAGCG	50.0	47.6	58.2	57.5	1408-1409 bp	1.0	66.5