Hotspot

petB

LSC · 78742-80145 · 1404 bp

Back to Sinojackia

Pi 0.000617

MarkerSeek score 50.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1404
variable_sites: 2
pi: 0.000617
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GAGCTCGGATTTTTAGCCCA	TGTTCCTCCCCCAAGTGATA	50.0	50.0	58.0	57.6	1527-1532 bp	1.0	77.2
petB_p2	GAGCTCGGATTTTTAGCCCA	ATTGTTCCTCCCCCAAGTGA	50.0	50.0	58.0	58.5	1529-1534 bp	1.0	75.7
petB_p3	GAGCTCGGATTTTTAGCCCA	TTGTTCCTCCCCCAAGTGAT	50.0	50.0	58.0	58.5	1528-1533 bp	1.0	75.7
petB_p4	AGAGCTCGGATTTTTAGCCC	TGTTCCTCCCCCAAGTGATA	50.0	50.0	57.7	57.6	1528-1533 bp	1.0	74.9
petB_p5	TAGTTCGACCGCGGAATTTC	TGTTCCTCCCCCAAGTGATA	50.0	50.0	58.4	57.6	1496-1501 bp	1.0	74.6