Hotspot

ndhH

SSC · 126153-127334 · 1182 bp

Back to Sinojackia

Pi 0.000733

MarkerSeek score 43.70

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 2
pi: 0.000733
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GGAACAAACATCCATATGATCCC	CGCCTTCAGCAATTCATGGA	43.5	50.0	57.3	58.9	1320 bp	1.0	43.2
ndhH_p2	GGAACAAACATCCATATGATCCC	TCGCCTTCAGCAATTCATGG	43.5	50.0	57.3	58.9	1321 bp	1.0	43.2
ndhH_p3	GGAACAAACATCCATATGATCCC	TTCGCCTTCAGCAATTCATG	43.5	45.0	57.3	57.1	1322 bp	1.0	43.0
ndhH_p4	GGAACAAACATCCATATGATCCC	GCCTTCAGCAATTCATGGAAG	43.5	47.6	57.3	57.8	1319 bp	1.0	41.1
ndhH_p5	GGAACAAACATCCATATGATCCC	TTTCGCCTTCAGCAATTCATG	43.5	42.9	57.3	57.8	1323 bp	1.0	40.7