Hotspot

ndhB-trnL-CAA

IRa · 148344-148897 · 554 bp

Back to Sinojackia

Pi 0
MarkerSeek score 34.70
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
554
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 ACAATTGCTCAGGATACCCT GTCTCGCGTGTCTACCATTT 45.0 50.0 56.2 58.0 613 bp 1.0 65.5
ndhB-trnL-CAA_p2 ACAATTGCTCAGGATACCCT CTCGCGTGTCTACCATTTCA 45.0 50.0 56.2 58.0 611 bp 1.0 65.5
ndhB-trnL-CAA_p3 ACAATTGCTCAGGATACCCT TACCATTTCACCACCAAGGC 45.0 50.0 56.2 58.1 601 bp 1.0 64.9
ndhB-trnL-CAA_p4 ACAATTGCTCAGGATACCCT GCGTGTCTACCATTTCACCA 45.0 50.0 56.2 58.2 608 bp 1.0 64.0
ndhB-trnL-CAA_p5 ACAATTGCTCAGGATACCCTT GTCTCGCGTGTCTACCATTT 42.9 50.0 57.0 58.0 613 bp 1.0 63.7