Hotspot

ndhF-trnL-UAG

SSC · 116374-118398 · 2025 bp

Back to Sinojackia

Pi 0.00372

MarkerSeek score 51.20

Primer available yes

Label ndhF-trnL-UAG

Feature Metadata

label_name: ndhF-trnL-UAG
feature_type: igs
region: SSC
length_bp: 2025
variable_sites: 13
pi: 0.00372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-trnL-UAG_p1	AAAAGAAGAAGCCCCACTCC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	2148-2150 bp	1.0	77.5
ndhF-trnL-UAG_p2	AAAAGAAGAAGCCCCACTCC	TTAGAAGATAGCATGCCGCC	50.0	50.0	57.7	57.8	2202-2204 bp	1.0	77.1
ndhF-trnL-UAG_p3	AAGAAGAAGCCCCACTCCTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	2146-2148 bp	1.0	77.1
ndhF-trnL-UAG_p4	AAAAGAAGAAGCCCCACTCC	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.7	57.8	2141-2143 bp	1.0	77.0
ndhF-trnL-UAG_p5	AAAAGAAGAAGCCCCACTCC	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.7	57.8	2146-2148 bp	1.0	76.9