Hotspot

trnN-GUU-ndhF

IRb · 112733-114132 · 1400 bp

Back to Sinojackia

Pi 0.00167

MarkerSeek score 44.50

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1400
variable_sites: 7
pi: 0.00167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGGATTCGAACCTACGACCA	ATAGGAGGTGGTCGCATCTC	50.0	55.0	58.2	58.7	1558-1607 bp	1.0	73.8
trnN-GUU-ndhF_p2	AGGATTCGAACCTACGACCA	TATAGGAGGTGGTCGCATCT	50.0	50.0	58.2	57.3	1559-1608 bp	1.0	73.7
trnN-GUU-ndhF_p3	AGGATTCGAACCTACGACCA	AGGTGGTCGCATCTCTTCTT	50.0	50.0	58.2	58.7	1553-1602 bp	1.0	73.3
trnN-GUU-ndhF_p4	AATCAGTTAACAGCCGACCG	ATAGGAGGTGGTCGCATCTC	50.0	55.0	58.3	58.7	1539-1588 bp	1.0	72.8
trnN-GUU-ndhF_p5	AATCAGTTAACAGCCGACCG	TATAGGAGGTGGTCGCATCT	50.0	50.0	58.3	57.3	1540-1589 bp	1.0	72.7