Hotspot

rpl16-rps3

LSC · 85452-86652 · 1201 bp

Back to Sinojackia

Pi 0.000944
MarkerSeek score 43.90
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1201
variable_sites
2
pi
0.000944
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 GGAATCTGGTTCTTTTGGGGT CCCTACAAACCATTCGAGCT 47.6 50.0 58.1 57.6 1335-1336 bp 1.0 68.1
rpl16-rps3_p2 CGGAATCTGGTTCTTTTGGG CCCTACAAACCATTCGAGCT 50.0 50.0 56.7 57.6 1336-1337 bp 1.0 66.5
rpl16-rps3_p3 GGAATCTGGTTCTTTTGGGGT GGGTTCCCCTACAAACCATT 47.6 50.0 58.1 57.4 1341-1342 bp 1.0 66.4
rpl16-rps3_p4 GGAATCTGGTTCTTTTGGGGT CCCCTACAAACCATTCGAGC 47.6 55.0 58.1 58.6 1336-1337 bp 1.0 66.3
rpl16-rps3_p5 ACGGAATCTGGTTCTTTTGGG CCCTACAAACCATTCGAGCT 47.6 50.0 58.5 57.6 1337-1338 bp 1.0 65.1