Hotspot

rbcL-accD

LSC · 59605-60751 · 1147 bp

Back to Sinojackia

Pi 0.00128

MarkerSeek score 53.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1147
variable_sites: 3
pi: 0.00128
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	TGTGACTACCACTTACAACGT	55.0	42.9	58.0	56.9	1338-1341 bp	1.0	63.3
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	TGTGACTACCACTTACAACGT	50.0	42.9	57.9	56.9	1308-1311 bp	1.0	62.7
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	TGTGACTACCACTTACAACGT	55.0	42.9	57.7	56.9	1324-1327 bp	1.0	61.3
rbcL-accD_p4	GGCTAGCAAATGGAGTCCTG	TGTGACTACCACTTACAACGT	55.0	42.9	58.3	56.9	1336-1339 bp	1.0	61.0
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	TTGTGACTACCACTTACAACGT	55.0	40.9	58.0	57.6	1339-1342 bp	1.0	60.9