Hotspot

rpoB-trnC-GCA

LSC · 27699-28809 · 1111 bp

Back to Sinojackia

Pi 0.00084

MarkerSeek score 43.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 2
pi: 0.00084
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCTTCATTTCCATCCCGGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1179 bp	1.0	74.0
rpoB-trnC-GCA_p2	TTCCTTCATTTCCATCCCGG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	58.4	1180 bp	1.0	73.8
rpoB-trnC-GCA_p3	TAAAACTCTTCCGCCAAGCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1262 bp	1.0	73.5
rpoB-trnC-GCA_p4	GCCAAGCCCTGATCAATGAA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.5	58.4	1250 bp	1.0	73.1
rpoB-trnC-GCA_p5	TCCTTCATTTCCATCCCGGA	GCGACACCCAGATTTGAACT	50.0	50.0	58.4	58.5	1204 bp	1.0	73.0