Hotspot

rps16-trnQ-UUG

LSC · 6074-7819 · 1746 bp

Back to Sinojackia

Pi 0.000878

MarkerSeek score 52.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1746
variable_sites: 4
pi: 0.000878
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1825-1827 bp	1.0	79.6
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1827-1829 bp	1.0	78.5
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1818-1820 bp	1.0	78.5
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1828-1830 bp	1.0	77.9
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	AAATTTAAATGGGGCGTGGC	50.0	45.0	58.0	57.6	1882-1884 bp	1.0	77.0