Pi
0.066
MarkerSeek score
82.30
Primer available
yes
Label
ycf15
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf15_p1 | AGTGGATGGGTGGTAGAGTC | GCCCGTAAACCAGCAGATAA | 55.0 | 50.0 | 58.1 | 58.0 | 252-277 bp | 1.0 | 84.8 |
| ycf15_p2 | CCGGAAAGATATGTGGCCAT | GCCCGTAAACCAGCAGATAA | 50.0 | 50.0 | 57.7 | 58.0 | 285-310 bp | 1.0 | 83.4 |
| ycf15_p3 | AGTGGATGGGTGGTAGAGTC | CCCCGAGTAGATGCTTCAAC | 55.0 | 55.0 | 58.1 | 58.1 | 282 bp | 0.5 | 58.5 |
| ycf15_p4 | AAACGCCCGTTTCTTCCATA | GCCCGTAAACCAGCAGATAA | 45.0 | 50.0 | 57.8 | 58.0 | 228 bp | 0.5 | 58.2 |
| ycf15_p5 | AAACGCCCGTTTCTTCCATA | CCCCGAGTAGATGCTTCAAC | 45.0 | 55.0 | 57.8 | 58.1 | 258 bp | 0.5 | 58.0 |
| ycf15_p1 | GCCCGTAAACCAGCAGATAA | AGTGGATGGGTGGTAGAGTC | 50.0 | 55.0 | 58.0 | 58.1 | 252-277 bp | 1.0 | 84.8 |
| ycf15_p2 | GCCCGTAAACCAGCAGATAA | CCGGAAAGATATGTGGCCAT | 50.0 | 50.0 | 58.0 | 57.7 | 285-310 bp | 1.0 | 83.4 |
| ycf15_p3 | CCCCGAGTAGATGCTTCAAC | AGTGGATGGGTGGTAGAGTC | 55.0 | 55.0 | 58.1 | 58.1 | 282 bp | 0.5 | 58.5 |
| ycf15_p4 | GCCCGTAAACCAGCAGATAA | AAACGCCCGTTTCTTCCATA | 50.0 | 45.0 | 58.0 | 57.8 | 228 bp | 0.5 | 58.2 |
| ycf15_p5 | CCCCGAGTAGATGCTTCAAC | AAACGCCCGTTTCTTCCATA | 55.0 | 45.0 | 58.1 | 57.8 | 258 bp | 0.5 | 58.0 |