Hotspot

ycf1

SSC · 106790-111994 · 5205 bp

Back to Sinogentiana

Pi 0.0271

MarkerSeek score 56.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5205
variable_sites: 140
pi: 0.0271
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCCCTTTTGCAATTTTGAGC	TTCCTTTTGGTTCGCCTCAA	42.9	45.0	58.2	57.9	1130-1151 bp	1.0	75.1
ycf1_p2	ACCCCTTTTGCAATTTTGAGC	TGCTAGGGCAATCACAATCC	42.9	50.0	58.2	57.9	775-781 bp	1.0	74.8
ycf1_p3	ACCCCTTTTGCAATTTTGAGC	CCTGAAACCTTGGCACAGAT	42.9	50.0	58.2	58.1	1243-1264 bp	1.0	74.7
ycf1_p4	ACCCCTTTTGCAATTTTGAGC	ATGTTCCTTTTGGTTCGCCT	42.9	45.0	58.2	58.0	1154 bp	0.5	50.8
ycf1_p5	AGAAATAGGCCTCAACAGCA	ATGTTCCTTTTGGTTCGCCT	45.0	45.0	56.8	58.0	1215 bp	0.5	50.5