Hotspot

ndhA

SSC · 103867-105171 · 1305 bp

Back to Sinogentiana

Pi 0.0278

MarkerSeek score 60.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1305
variable_sites: 36
pi: 0.0278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	AACCAACGCAGTCTTCTGTT	AGAGTTGAAGCCCCAAAAGG	45.0	50.0	58.0	58.0	1886 bp	0.5	59.7
ndhA_p2	ACCAGACGAAGCAACAAGTT	AGAGTTGAAGCCCCAAAAGG	45.0	50.0	58.0	58.0	1664 bp	0.5	59.7
ndhA_p3	AACCAACGCAGTCTTCTGTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.0	58.0	1889 bp	0.5	59.5
ndhA_p4	ACCAGACGAAGCAACAAGTT	GTGAGAGTTGAAGCCCCAAA	45.0	50.0	58.0	58.0	1667 bp	0.5	59.5
ndhA_p5	ACCAGACGAAGCAACAAGTT	GGGGTAGCTGCTGATTTACC	45.0	55.0	58.0	58.0	2141 bp	0.5	59.4