Hotspot

rrn5-rrn4.5

IRa · 113149-113335 · 187 bp

Back to Sinogentiana

Pi 0.165

MarkerSeek score 77.20

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 187
variable_sites: 28
pi: 0.165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	CTCTAAGAAGGGTGCGTTCC	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	462-494 bp	1.0	87.4
rrn5-rrn4.5_p2	CTCTAAGAAGGGTGCGTTCC	GTCAAGTGGAAGTGCAGTGA	55.0	50.0	58.0	58.0	488-520 bp	1.0	87.0
rrn5-rrn4.5_p3	CTCTAAGAAGGGTGCGTTCC	ACCTGGGGCTGTAGTATGTT	55.0	50.0	58.0	58.0	902-934 bp	1.0	83.8
rrn5-rrn4.5_p4	CTCTAAGAAGGGTGCGTTCC	TGGGGCTGTAGTATGTTCCA	55.0	50.0	58.0	58.0	899-931 bp	1.0	83.8
rrn5-rrn4.5_p5	CTCTAAGAAGGGTGCGTTCC	TAACAGGCTGATCTTCCCCA	55.0	50.0	58.0	58.0	972-1004 bp	1.0	83.5