Hotspot

trnN-GUU-trnR-ACG

IRa · 112398-112722 · 325 bp

Back to Sinogentiana

Pi 0.165

MarkerSeek score 76.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 325
variable_sites: 45
pi: 0.165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	GGAACGCACCCTTCTTAGAG	55.0	55.0	58.0	58.0	611-636 bp	1.0	89.8
trnN-GUU-trnR-ACG_p2	CCGTTGTTCCTCAGTAGCTC	CAGCTGAGGCATCCTAACAG	55.0	55.0	58.0	58.0	1053-1110 bp	1.0	89.2
trnN-GUU-trnR-ACG_p3	CCGTTGTTCCTCAGTAGCTC	GTCAAGTGGAAGTGCAGTGA	55.0	50.0	58.0	58.0	1079-1136 bp	1.0	89.1
trnN-GUU-trnR-ACG_p4	CACTACGAAGAAGTTCCGGG	GGAACGCACCCTTCTTAGAG	55.0	55.0	58.0	58.0	725 bp	0.5	59.8
trnN-GUU-trnR-ACG_p5	CCACTACGAAGAAGTTCCGG	GGAACGCACCCTTCTTAGAG	55.0	55.0	58.0	58.0	726 bp	0.5	59.8