Hotspot

trnR-ACG-trnN-GUU

IRb · 95944-96268 · 325 bp

Back to Sinogentiana

Pi 0.165

MarkerSeek score 76.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 325
variable_sites: 45
pi: 0.165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GGAACGCACCCTTCTTAGAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.0	58.0	611-636 bp	1.0	89.8
trnR-ACG-trnN-GUU_p2	CAGCTGAGGCATCCTAACAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	58.0	58.0	1053-1110 bp	1.0	89.2
trnR-ACG-trnN-GUU_p3	GTCAAGTGGAAGTGCAGTGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.0	58.0	1079-1136 bp	1.0	89.1
trnR-ACG-trnN-GUU_p4	GGAACGCACCCTTCTTAGAG	CACTACGAAGAAGTTCCGGG	55.0	55.0	58.0	58.0	725 bp	0.5	59.8
trnR-ACG-trnN-GUU_p5	GGAACGCACCCTTCTTAGAG	CCACTACGAAGAAGTTCCGG	55.0	55.0	58.0	58.0	726 bp	0.5	59.8