Hotspot

petA-psbJ

LSC · 55479-55941 · 463 bp

Back to Sinogentiana

Pi 0.0552

MarkerSeek score 79.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 463
variable_sites: 24
pi: 0.0552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGGCTGA	ATTCCGGATTAGGGTCATCC	42.9	50.0	57.5	56.5	518-523 bp	1.0	63.2
petA-psbJ_p2	TGAGAAGGTTCAATTGGCTG	ATTCCGGATTAGGGTCATCC	45.0	50.0	55.9	56.5	518-523 bp	1.0	58.8
petA-psbJ_p3	TGAGAAGGTTCAATTGGCTGAA	ATTCCGGATTAGGGTCATCC	40.9	50.0	58.2	56.5	518-523 bp	1.0	57.9
petA-psbJ_p4	TTGAGAAGGTTCAATTGGCTGA	ATTCCGGATTAGGGTCATCC	40.9	50.0	58.2	56.5	519-524 bp	1.0	57.9
petA-psbJ_p5	TTGAGAAGGTTCAATTGGCTG	ATTCCGGATTAGGGTCATCC	42.9	50.0	56.7	56.5	519-524 bp	1.0	57.0