Hotspot

trnC-GCA-petN

LSC · 24860-25551 · 692 bp

Back to Sinogentiana

Pi 0.0423

MarkerSeek score 81.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 692
variable_sites: 27
pi: 0.0423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 81.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	733-755 bp	1.0	86.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	727-749 bp	1.0	84.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	751-773 bp	1.0	83.9
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TAGTCCACTTCTTCCCCACA	50.0	50.0	58.1	57.6	786-808 bp	1.0	82.8
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	TCCACTTCTTCCCCACACTA	50.0	50.0	58.1	57.6	783-805 bp	1.0	82.8