Hotspot

trnK-UUU-trnQ-UUG

LSC · 4100-4841 · 742 bp

Back to Sinogentiana

Pi 0.0634

MarkerSeek score 77.60

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 742
variable_sites: 42
pi: 0.0634
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CGAATCCCTTGCTTCATCCA	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1381-1417 bp	1.0	86.8
trnK-UUU-trnQ-UUG_p2	CGAATCCCTTGCTTCATCCA	CTTACAGCAGCTTGCCAAAC	50.0	50.0	58.0	57.9	1386-1422 bp	1.0	86.7
trnK-UUU-trnQ-UUG_p3	ACGAATCCCTTGCTTCATCC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1382-1418 bp	1.0	86.7
trnK-UUU-trnQ-UUG_p4	ATGGACGAATCCCTTGCTTC	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1386-1422 bp	1.0	86.7
trnK-UUU-trnQ-UUG_p5	GGACGAATCCCTTGCTTCAT	AGCAGCTTGCCAAACAAAAG	50.0	45.0	58.0	58.1	1384-1420 bp	1.0	86.7