Hotspot

ndhK

LSC · 48832-49599 · 768 bp

Back to Sinocrassula

Pi 0

MarkerSeek score 61.40

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 768
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AAAATGGACCCACTGCTTGT	TCCATGGGCAATGAGTTTTGA	45.0	42.9	57.9	58.1	883-904 bp	1.0	72.3
ndhK_p2	AAATGGACCCACTGCTTGTT	TCCATGGGCAATGAGTTTTGA	45.0	42.9	57.9	58.1	882-903 bp	1.0	72.3
ndhK_p3	AAAATGGACCCACTGCTTGT	TGATGTAGTGGGTGTATCCGT	45.0	47.6	57.9	58.3	865-886 bp	1.0	71.2
ndhK_p4	AAATGGACCCACTGCTTGTT	TGATGTAGTGGGTGTATCCGT	45.0	47.6	57.9	58.3	864-885 bp	1.0	71.2
ndhK_p5	TGAAAATGGACCCACTGCTT	TCCATGGGCAATGAGTTTTGA	45.0	42.9	57.6	58.1	885-906 bp	1.0	69.9