Hotspot

rpl2-rps19

IRa · 151610-151663 · 54 bp

Back to Sinocrassula

Pi 0.0185

MarkerSeek score 61.90

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: IRa
length_bp: 54
variable_sites: 1
pi: 0.0185
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	GGGCCCCAATTGGTAGAAAA	GCCCGGGACCAAGTTACTAT	50.0	55.0	58.1	59.2	283 bp	1.0	70.5
rpl2-rps19_p2	AGAAAAAGACCCACAACCCC	GCCCGGGACCAAGTTACTAT	50.0	55.0	57.9	59.2	269 bp	1.0	70.3
rpl2-rps19_p3	GAAAAAGACCCACAACCCCT	GCCCGGGACCAAGTTACTAT	50.0	55.0	57.9	59.2	268 bp	1.0	70.3
rpl2-rps19_p4	TTGATTCTTCGTCGCCGTAG	GCCCGGGACCAAGTTACTAT	50.0	55.0	58.1	59.2	191 bp	1.0	70.3
rpl2-rps19_p5	CACAACCCCTTGGGGTTATC	GCCCGGGACCAAGTTACTAT	55.0	55.0	58.2	59.2	258 bp	1.0	69.8