Hotspot

ndhF-rpl32

SSC · 111164-111773 · 610 bp

Back to Sinocrassula

Pi 0.00174

MarkerSeek score 64.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 610
variable_sites: 1
pi: 0.00174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTTTTTGTTGCCGTCGGAAA	TTAACGCTGCCCAATATCCC	45.0	50.0	57.8	58.0	772-802 bp	1.0	81.9
ndhF-rpl32_p2	CTTTTTGTTGCCGTCGGAAA	AAAAGGCTTTTAACGCTGCC	45.0	45.0	57.8	57.9	781-811 bp	1.0	80.9
ndhF-rpl32_p3	AAGACTTTTTGTTGCCGTCG	TTAACGCTGCCCAATATCCC	45.0	50.0	57.5	58.0	776-806 bp	1.0	79.7
ndhF-rpl32_p4	CGAAGACTTTTTGTTGCCGT	TTAACGCTGCCCAATATCCC	45.0	50.0	57.5	58.0	778-808 bp	1.0	79.7
ndhF-rpl32_p5	AAGACTTTTTGTTGCCGTCG	AAAAGGCTTTTAACGCTGCC	45.0	45.0	57.5	57.9	785-815 bp	1.0	78.7