Hotspot

psaJ-rpl33

LSC · 66204-66659 · 456 bp

Back to Sinocrassula

Pi 0.00696

MarkerSeek score 64.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 3
pi: 0.00696
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AGATGCGCTGACATTTCCTT	TCGTACATCTTTACTCTTGGCC	45.0	45.5	57.9	57.9	489-514 bp	1.0	66.6
psaJ-rpl33_p2	AGATGCGCTGACATTTCCTT	CGTACATCTTTACTCTTGGCCA	45.0	45.5	57.9	58.1	488-513 bp	1.0	66.4
psaJ-rpl33_p3	TGTGGCACCGGTACTAAGTA	TCGTACATCTTTACTCTTGGCC	50.0	45.5	58.1	57.9	564-589 bp	1.0	66.3
psaJ-rpl33_p4	TGTGGCACCGGTACTAAGTA	CGTACATCTTTACTCTTGGCCA	50.0	45.5	58.1	58.1	563-588 bp	1.0	66.1
psaJ-rpl33_p5	TGTGGCACCGGTACTAAGTA	TCTGGAAATACCCCTTGAGTCT	50.0	45.5	58.1	58.2	636-661 bp	1.0	65.3