Hotspot

ycf4-cemA

LSC · 59561-60210 · 650 bp

Back to Sinocrassula

Pi 0.00163

MarkerSeek score 64.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 650
variable_sites: 1
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CCTATTTCTTGCGCGTACCT	AGATCAACCCGGGCAAAAAT	50.0	45.0	58.1	58.1	718-755 bp	1.0	83.1
ycf4-cemA_p2	TGACTCCACGCGAAATTGAA	AGATCAACCCGGGCAAAAAT	45.0	45.0	58.1	58.1	757-794 bp	1.0	82.4
ycf4-cemA_p3	TTGACTCCACGCGAAATTGA	AGATCAACCCGGGCAAAAAT	45.0	45.0	58.1	58.1	758-795 bp	1.0	82.4
ycf4-cemA_p4	AGAATTTGACTCCACGCGAA	AGATCAACCCGGGCAAAAAT	45.0	45.0	57.8	58.1	763-800 bp	1.0	82.1
ycf4-cemA_p5	AAAAAGCTGCTGAATTGGCC	AGATCAACCCGGGCAAAAAT	45.0	45.0	57.8	58.1	736-773 bp	1.0	82.1