Hotspot

rbcL-accD

LSC · 55944-56618 · 675 bp

Back to Sinocrassula

Pi 0.00619

MarkerSeek score 62.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 4
pi: 0.00619
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	AATTGAACCGCCGTTTTTCC	55.0	45.0	57.7	57.9	744-773 bp	1.0	80.0
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	AGGCCCAAGATTGTCCATTG	55.0	50.0	57.7	58.1	812-841 bp	1.0	79.7
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	GGCCCAAGATTGTCCATTGA	55.0	50.0	57.7	58.1	811-840 bp	1.0	79.7
rbcL-accD_p4	GAGTCCTGAACTAGCTGCTG	CGAATTGAACCGCCGTTTTT	55.0	45.0	57.7	58.2	746-775 bp	1.0	79.2
rbcL-accD_p5	GCTGCTGCTTGTGAAGTATG	AATTGAACCGCCGTTTTTCC	50.0	45.0	57.5	57.9	731-760 bp	1.0	78.1