Hotspot

psbZ-trnG-UCC

LSC · 36200-36826 · 627 bp

Back to Sinocrassula

Pi 0.0901

MarkerSeek score 80.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 627
variable_sites: 52
pi: 0.0901
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GTTTCCGTTTTTCTGGCTCG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1560-1801 bp	1.0	84.8
psbZ-trnG-UCC_p2	TTTTCTGGCTCGGCTATTCC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	1552-1793 bp	1.0	84.7
psbZ-trnG-UCC_p3	CAGCCATCTCTCCGAAAGAC	CGTTGTTTTTCGACCGGAAG	55.0	50.0	58.1	58.0	1715-1956 bp	1.0	84.2
psbZ-trnG-UCC_p4	GTTTCCGTTTTTCTGGCTCG	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1896-2137 bp	1.0	84.1
psbZ-trnG-UCC_p5	TTTTCTGGCTCGGCTATTCC	CGTTGTTTTTCGACCGGAAG	50.0	50.0	58.0	58.0	1888-2129 bp	1.0	84.0