Hotspot

trnS-GCU-trnG-GCC

LSC · 8308-8913 · 606 bp

Back to Sinocrassula

Pi 0.00831

MarkerSeek score 56.40

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 606
variable_sites: 5
pi: 0.00831
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	729-731 bp	1.0	56.2
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.0	57.9	728-730 bp	1.0	56.2
trnS-GCU-trnG-GCC_p3	GCTCGTACAACGGATTAGCA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.1	57.9	747-749 bp	1.0	55.5
trnS-GCU-trnG-GCC_p4	ACGGAAAGAGAGGGATTCGA	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.2	57.9	783-785 bp	1.0	54.9
trnS-GCU-trnG-GCC_p5	GAATCGCTCGTACAACGGAT	TGTTCGGAATACAAAGAAAACCG	50.0	39.1	58.2	57.9	752-754 bp	1.0	54.9